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IsoSeq contains the newest tools to identify transcripts in PacBio single-molecule sequencing data. Starting in SMRT Link v6.0.0, those tools power the Iso-Seq GUI-based analysis application. A composable workflow of existing tools and algorithms, combined with new clustering techniques, allows to process the ever-increasing yield of PacBio machines. Starting with version 3.4, support for UMI and cell barcode based deduplication has been added. Version 4.0 adds a new cluster2 tool that enables clustering of hundreds of millions of HiFi reads.

Availability

Latest version can be installed via bioconda package isoseq.

Please refer to our official pbbioconda page for information on Installation, Support, License, Copyright, and Disclaimer.

Latest Version

Version 4.2.0: Full changelog here

What’s new!

Version 4.0 adds a new cluster2 tool that enables clustering of hundreds of millions of HiFi reads. This is an early access version. Additional documentation will follow soon.

The binary has been renamed from isoseq3 to isoseq to enable major version changes. Bioconda will still generate a isoseq3 softlink. The old bioconda isoseq3 package will automatically install the latest isoseq package.

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