Iso-Seq contains the newest tools to identify transcripts in PacBio single-molecule sequencing data. Starting in SMRT Link v6.0.0, those tools power the Iso-Seq GUI-based analysis application. A composable workflow of existing tools and algorithms, combined with a new clustering technique, allows to process the ever-increasing yield of PacBio. Starting with version 3.4, support for UMI and cell barcode based deduplication has been added.
Latest version can be installed via bioconda package
Please refer to our official pbbioconda page for information on Installation, Support, License, Copyright, and Disclaimer.
Version 3.4.0: Full changelog here
New documentation is up, a 1:1 port from the original GitHub docs with minor enhancements.
If you are new to demultiplexing barcoded samples, check out the Get Started guide.