Isoform Categories

Pigeon follows the SQANTI3 classification categories.

Category Description
FSM (Full Splice Match) The reference and query isoform have the same number of exons and each internal junction matches the positions of the reference. The exact 5’ start and 3’ end can differ within the first/last exons.
ISM (Incomplete Splice Match) The query isoform has fewer external exons than the reference, but each internal junction matches the positions of the reference. The exact 5’ start and 3’ end can differ within the first/last exons.
NIC (Novel In Catalog) The query isoform does not have a FSM or ISM match, but is using a combination of known donor/acceptor sites.
NNC (Novel Not in Catalog) The query isoform does not have a FSM or ISM match, and has at least one donor or acceptor site that is not annotated.
Antisense The query isoform does not have overlap a same-strand reference gene but is anti-sense to an annotated gene.
Genic Intron The query isoform is completely contained within a reference intron.
Genic Genomic The query isoform overlaps with introns and exons.
Intergenic The query isoform is in the intergenic region.

Category Overview

isoform categories

Subcategories

Additionally there are categories that are divided into subcategories.

fsm subcategories

ism subcategories

nnc nic subcategories

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