Nomenclature

CCS reads

Circular Consensus Sequencing (CCS) reads generated by the CCS algorithm

HiFi reads

CCS Reads with predicted accuracy ≥ Q20

S-reads

Segmented reads (S-reads) are CCS Reads that have been segmented by Skera based on the presence of segmentation adapters

Skera

Tool for segmenting CCS reads based on the presence of segmentation adapters

Lima

Tool for demultiplex and remove PacBio barcodes and primers

UMI

Unique Molecular Identifier